April Pyle, Ph.D.
Professor and Vice Chair, Microbiology, Immunology & Molecular Genetics Member, CTSI, Cell & Developmental Biology GPB Home Area, Center for Duchenne Muscular Dystrophy, Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, JCCC Cancer and Stem Cell Biology Program Area
apyle@mednet.ucla.edu
310-794-4059
Laboratory Address:
615 Charles E. Young Dr. South
Los Angeles , CA 90095
Work Address:
615 Charles E. Young Dr. South
Los Angeles , CA 90095
Biography
Dr. Pyle received her Ph.D. from the University of Tennessee in 2002 and completed her postdoctoral fellowship work with Peter Donovan in 2006 at Johns Hopkins University. She is currently a Professor in the Department of Microbiology, Immunology and Molecular Genetics at UCLA and a member of the Eli and Edythe Broad Stem Cell Center, the Center for Duchenne Muscular Dystrophy and the Jonsson Comprehensive Cancer Center at UCLA. Dr. Pyle’s lab uses multi-disciplinary approaches to study human pluripotent stem cell biology and differentiation of these cells for use in regenerative medicine. Dr. Pyle’s lab studies both basic aspects of stem cell biology as well as more translational aspects of human pluripotent stem cell differentiation towards skeletal muscle for use in therapeutic approaches for patients with muscular dystrophy.
Publications
A selected list of publications:
Hicks Michael R, Hiserodt Julia, Paras Katrina, Fujiwara Wakana, Eskin Ascia, Jan Majib, Xi Haibin, Young Courtney S, Evseenko Denis, Nelson Stanley F, Spencer Melissa J, Handel Ben Van, Pyle April D ERBB3 and NGFR mark a distinct skeletal muscle progenitor cell in human development and hPSCs Nature cell biology, 2017; .
Young Courtney S, Mokhonova Ekaterina, Quinonez Marbella, Pyle April D, Spencer Melissa J Creation of a Novel Humanized Dystrophic Mouse Model of Duchenne Muscular Dystrophy and Application of a CRISPR/Cas9 Gene Editing Therapy Journal of neuromuscular diseases, 2017; 4(2): 139-145.
Hazim Roni A, Karumbayaram Saravanan, Jiang Mei, Dimashkie Anupama, Lopes Vanda S, Li Douran, Burgess Barry L, Vijayaraj Preethi, Alva-Ornelas Jackelyn A, Zack Jerome A, Kohn Donald B, Gomperts Brigitte N, Pyle April D, Lowry William E, Williams David S Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization Stem cell research & therapy, 2017; 8(1): 217.
Xi Haibin, Fujiwara Wakana, Gonzalez Karen, Jan Majib, Liebscher Simone, Van Handel Ben, Schenke-Layland Katja, Pyle April D In Vivo Human Somitogenesis Guides Somite Development from hPSCs Cell reports, 2017; 18(6): 1573-1585.
Young Courtney S, Hicks Michael R, Ermolova Natalia V, Nakano Haruko, Jan Majib, Younesi Shahab, Karumbayaram Saravanan, Kumagai-Cresse Chino, Wang Derek, Zack Jerome A, Kohn Donald B, Nakano Atsushi, Nelson Stanley F, Miceli M Carrie, Spencer Melissa J, Pyle April D A Single CRISPR-Cas9 Deletion Strategy that Targets the Majority of DMD Patients Restores Dystrophin Function in hiPSC-Derived Muscle Cells Cell stem cell, 2016; 18(4): 533-40.
Young Courtney S, Pyle April D Exon Skipping Therapy Cell, 2016; 167(5): 1144.
Lee Patrick C, Truong Brian, Vega-Crespo Agustin, Gilmore W Blake, Hermann Kip, Angarita Stephanie Ak, Tang Jonathan K, Chang Katherine M, Wininger Austin E, Lam Alex K, Schoenberg Benjamen E, Cederbaum Stephen D, Pyle April D, Byrne James A, Lipshutz Gerald S Restoring Ureagenesis in Hepatocytes by CRISPR/Cas9-mediated Genomic Addition to Arginase-deficient Induced Pluripotent Stem Cells Molecular therapy. Nucleic acids, 2016; 5(11): e394.
Thakore-Shah K, Koleilat T, Jan M, John A, Pyle AD. REST/NRSF Knockdown Alters Survival, Lineage Differentiation and Signaling in Human Embryonic Stem Cells, Plos One, 2015; 10(12): .
Hou Shuang, Choi Jin-sil, Chen Kuan-Ju, Zhang Yang, Peng Jinliang, Garcia Mitch A, Yu Jue-Hua, Thakore-Shah Kaushali, Ro Tracy, Chen Jie-Fu, Peyda Parham, Fan Guoping, Pyle April D, Wang Hao, Tseng Hsian-Rong Supramolecular nanosubstrate-mediated delivery for reprogramming and transdifferentiation of mammalian cells Small (Weinheim an der Bergstrasse, Germany), 2015; 11(21): 2499-504.
Hicks M, Pyle A. The Path from Pluripotency to Skeletal Muscle: Developmental Myogenesis Guides the Way, Cell Stem Cell, 2015; 17(3): 255-7.
Peng Jinliang, Garcia Mitch André, Choi Jin-sil, Zhao Libo, Chen Kuan-Ju, Bernstein James R, Peyda Parham, Hsiao Yu-Sheng, Liu Katherine W, Lin Wei-Yu, Pyle April D, Wang Hao, Hou Shuang, Tseng Hsian-Rong Molecular recognition enables nanosubstrate-mediated delivery of gene-encapsulated nanoparticles with high efficiency ACS nano, 2014; 8(5): 4621-9.
Jonas Steven J, Alva Jackelyn A, Richardson Wade, Sherman Sean P, Galic Zoran, Pyle April D, Dunn Bruce A spatially and chemically defined platform for the uniform growth of human pluripotent stem cells Materials science & engineering. C, Materials for biological applications, 2013; 33(1): 234-41.
Awe Jason P, Lee Patrick C, Ramathal Cyril, Vega-Crespo Agustin, Durruthy-Durruthy Jens, Cooper Aaron, Karumbayaram Saravanan, Lowry William E, Clark Amander T, Zack Jerome A, Sebastiano Vittorio, Kohn Donald B, Pyle April D, Martin Martin G, Lipshutz Gerald S, Phelps Patricia E, Pera Renee A Reijo, Byrne James A Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status Stem cell research & therapy, 2013; 4(4): 87.
Yanes Rolando E, Tarn Derrick, Hwang Angela A, Ferris Daniel P, Sherman Sean P, Thomas Courtney R, Lu Jie, Pyle April D, Zink Jeffrey I, Tamanoi Fuyuhiko Involvement of lysosomal exocytosis in the excretion of mesoporous silica nanoparticles and enhancement of the drug delivery effect by exocytosis inhibition Small (Weinheim an der Bergstrasse, Germany), 2013; 9(5): 697-704.
Palomares Karina, Vigant Frederic, Van Handel Ben, Pernet Olivier, Chikere Kelechi, Hong Patrick, Sherman Sean P, Patterson Michaela, An Dong Sung, Lowry William E, Mikkola Hanna K A, Morizono Kouki, Pyle April D, Lee Benhur Nipah virus envelope-pseudotyped lentiviruses efficiently target ephrinB2-positive stem cell populations in vitro and bypass the liver sink when administered in vivo Journal of virology, 2013; 87(4): 2094-108.
Sherman Sean P, Pyle April D Small molecule screening with laser cytometry can be used to identify pro-survival molecules in human embryonic stem cells PloS one, 2013; 8(1): e54948.
Karumbayaram Saravanan, Lee Peiyee, Azghadi Soheila F, Cooper Aaron R, Patterson Michaela, Kohn Donald B, Pyle April, Clark Amander, Byrne James, Zack Jerome A, Plath Kathrin, Lowry William E From skin biopsy to neurons through a pluripotent intermediate under Good Manufacturing Practice protocols Stem cells translational medicine, 2012; 1(1): 36-43.
Sherman Sean P, Alva Jackelyn A, Thakore-Shah Kaushali, Pyle April D Human pluripotent stem cells: the development of high-content screening strategies Methods in molecular biology (Clifton, N.J.), 2011; 767(1): 283-95.
Alva Jackelyn A, Lee Grace E, Escobar Erika E, Pyle April D Phosphatase and tensin homolog regulates the pluripotent state and lineage fate choice in human embryonic stem cells Stem cells (Dayton, Ohio), 2011; 29(12): 1952-62.
Le Belle Janel E, Orozco Nicolas M, Paucar Andres A, Saxe Jonathan P, Mottahedeh Jack, Pyle April D, Wu Hong, Kornblum Harley I Proliferative neural stem cells have high endogenous ROS levels that regulate self-renewal and neurogenesis in a PI3K/Akt-dependant manner Cell stem cell, 2011; 8(1): 59-71.
Tchieu Jason, Kuoy Edward, Chin Mark H, Trinh Hung, Patterson Michaela, Sherman Sean P, Aimiuwu Otaren, Lindgren Anne, Hakimian Shahrad, Zack Jerome A, Clark Amander T, Pyle April D, Lowry William E, Plath Kathrin Female human iPSCs retain an inactive X chromosome Cell stem cell, 2010; 7(3): 329-42.
Tseng Han-Ching, Arasteh Aida, Paranjpe Avina, Teruel Antonia, Yang Wendy, Behel Armin, Alva Jackelyn A, Walter Gina, Head Christian, Ishikawa Tomo-o, Herschman Harvey R, Cacalano Nicholas, Pyle April D, Park No-Hee, Jewett Anahid Increased lysis of stem cells but not their differentiated cells by natural killer cells; de-differentiation or reprogramming activates NK cells PloS one, 2010; 5(7): e11590.
Kamei Ken-ichiro, Ohashi Minori, Gschweng Eric, Ho Quinn, Suh Jane, Tang Jinghua, For Yu Zeta Tak, Clark Amander T, Pyle April D, Teitell Michael A, Lee Ki-Bum, Witte Owen N, Tseng Hsian-Rong Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions Lab on a chip, 2010; 10(9): 1113-9.
Conway Anne E, Lindgren Anne, Galic Zoran, Pyle April D, Wu Hong, Zack Jerome A, Pelligrini Matteo, Teitell Michael A, Clark Amander T A self-renewal program controls the expansion of genetically unstable cancer stem cells in pluripotent stem cell-derived tumors Stem cells (Dayton, Ohio), 2009; 27(1): 18-28.
Chin Mark H, Mason Mike J, Xie Wei, Volinia Stefano, Singer Mike, Peterson Cory, Ambartsumyan Gayane, Aimiuwu Otaren, Richter Laura, Zhang Jin, Khvorostov Ivan, Ott Vanessa, Grunstein Michael, Lavon Neta, Benvenisty Nissim, Croce Carlo M, Clark Amander T, Baxter Tim, Pyle April D, Teitell Mike A, Pelegrini Matteo, Plath Kathrin, Lowry William E Induced pluripotent stem cells and embryonic stem cells are distinguished by gene expression signatures Cell stem cell, 2009; 5(1): 111-23.
Damoiseaux Robert, Sherman Sean P, Alva Jackelyn A, Peterson Cory, Pyle April D Integrated chemical genomics reveals modifiers of survival in human embryonic stem cells Stem cells (Dayton, Ohio), 2009; 27(3): 533-42.
Zeng Weihua, de Greef Jessica C, Chen Yen-Yun, Chien Richard, Kong Xiangduo, Gregson Heather C, Winokur Sara T, Pyle April, Robertson Keith D, Schmiesing John A, Kimonis Virginia E, Balog Judit, Frants Rune R, Ball Alexander R, Lock Leslie F, Donovan Peter J, van der Maarel Silvère M, Yokomori Kyoko Specific loss of histone H3 lysine 9 trimethylation and HP1gamma/cohesin binding at D4Z4 repeats is associated with facioscapulohumeral dystrophy (FSHD) PLoS genetics, 2009; 5(7): e1000559.
Wu Hao, Kim Kevin J, Mehta Kshama, Paxia Salvatore, Sundstrom Andrew, Anantharaman Thomas, Kuraishy Ali I, Doan Tri, Ghosh Jayati, Pyle April D, Clark Amander, Lowry William, Fan Guoping, Baxter Tim, Mishra Bud, Sun Yi, Teitell Michael A Copy number variant analysis of human embryonic stem cells Stem cells (Dayton, Ohio), 2008; 26(6): 1484-9.
Hohenstein Kristi A, Pyle April D, Chern Jing Yi, Lock Leslie F, Donovan Peter J Nucleofection mediates high-efficiency stable gene knockdown and transgene expression in human embryonic stem cells Stem cells (Dayton, Ohio), 2008; 26(6): 1436-43.
Dravid Gautam, Ye Zhaohui, Hammond Holly, Chen Guibin, Pyle April, Donovan Peter, Yu Xiaobing, Cheng Linzhao Defining the role of Wnt/beta-catenin signaling in the survival, proliferation, and self-renewal of human embryonic stem cells Stem cells (Dayton, Ohio), 2006; 23(10): 1489-501.
Pyle April D, Lock Leslie F, Donovan Peter J Neurotrophins mediate human embryonic stem cell survival Nature biotechnology, 2006; 24(3): 344-50.
Fenderson Bruce A, De Miguel Maria P, Pyle April D, Donovan Peter J Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens Methods in molecular biology (Clifton, N.J.), 2006; 325(3): 207-24.
Sharan Shyam K, Pyle April, Coppola Vincenzo, Babus Janice, Swaminathan Srividya, Benedict Jamie, Swing Deborah, Martin Betty K, Tessarollo Lino, Evans Janice P, Flaws Jodi A, Handel Mary Ann BRCA2 deficiency in mice leads to meiotic impairment and infertility Development (Cambridge, England), 2004; 131(1): 131-42.
Pyle April D, Donovan Peter J, Lock Leslie F Chipping away at ‘stemness’ Genome biology, 2004; 5(8): 235.
Russell Liane B, Hunsicker Patricia R, Kerley Marilyn, Pyle April, Saxton Arnold M Etoposide exposure during male mouse pachytene has complex effects on crossing-over and causes nondisjunction Mutation research, 2004; 565(1): 61-77.
Pyle April, Handel Mary Ann Meiosis in male PL/J mice: a genetic model for gametic aneuploidy Molecular reproduction and development, 2003; 64(4): 471-81.
Eaker Shannon, Cobb John, Pyle April, Handel Mary Ann Meiotic prophase abnormalities and metaphase cell death in MLH1-deficient mouse spermatocytes: insights into regulation of spermatogenic progress Developmental biology, 2002; 249(1): 85-95.